Amiloride analog stimulation of short-circuit current in larval frog skin epithelium.
نویسندگان
چکیده
منابع مشابه
Basal and amiloride-induced short-circuit current across isolated toad skin (Bufo arenarum).
We have previously demonstrated that amiloride (amil) addition to the isolated ventral pelvic (VPel) skin of Bufo arenarum toad induces negative short-circuit current values, which are equivalent to the isotopically measured net chloride transport. In the present work, we found that exposure of various regions of toad skin to amil yielded different values of short-circuit current (aSCC): negati...
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The apparent association constants of two agents, amiloride and triamterene, that block the Na-selective channel of apical membrane of frog skin are shown to decrease as the Na concentration is increased in the apical bathing solution in isolated skin of the frog, Rana temporaria, Rana esculenta, and Rana pipiens. These results were obtained in "normally polarized" skins. These effects were ind...
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In isolated skins of Leptodactylus ocellatus the short-circuit current is smaller than the sodium net flux and this difference disappears when the skins are bathed in solutions in which the chloride ions have been replaced by sulfate or methylsulfate ions. There is a net movement of chloride ions from outside to inside of the skins in the short-circuit condition with chloride Ringer's solutions...
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In the frog retinal pigment epithelium (RPE), the cellular levels ofcyclic AMP (CAMP) were measured in control conditions and after treatment with substances that are known to inhibit phosphodiesterase (PDE) activity (isobutyl-l-methylxanthine, SQ65442) or stimulate adenylate cyclase activity (forskolin) . The CAMP levels were elevated by a factor of 5-7 compared with the controls in PDE-treate...
متن کاملLocalization of Permeability Barriers in the Frog Skin Epithelium
Ruthenium red and colloidal lanthanum were used to determine the site of the structural barriers to diffusion within the intercellular spaces of frog skin epithelium. Electron micrographs show that occluding zonules located at the outer border of the stratum corneum and at the outer layer of the stratum granulosum are true tight junctions since they are impermeable to these tracers. Measurement...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 1997
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.200.23.3055